Analysis of Apoptosis-Related Genes in Hepatocellular Carcinoma Cells
肝癌细胞凋亡相关基因分析

摘要

目的 探讨分析肝癌细胞的凋亡基因，并分析相应的基因序列，探讨三氧化二砷对肝癌细胞凋亡产生分子机制。方法 本次研究在实验时采用三氧化二砷对人肝癌HCC-9204细胞进行诱导凋亡，并采用抑制性消减杂交技术，对凋亡细胞中的差异性表达，cDNA进行克隆，针对已知基因序列进行对比分析同源性特点。结果 在本次研究结果中，克隆到18个与已知基因有高度同源性的cdna序列其中的基因组控制类型包括抗氧化损伤、线粒体跨膜运输细胞骨架以及系统相关基因。结论 三氧化二砷诱导的肝癌HCC-9204细胞出现凋亡后，大量的基因表达出现上调的特征，而这些基因与三氧化二砷处理肝癌系细胞后的应激反应与细胞凋亡有密切的关联性，针对本次研究结果进行判断，提示细胞凋亡具有多基因参与的特征，受到诸多基因类型的调控是一个极为复杂的过程。

Abstract

Objective To explore and analyze the apoptosis genes of liver cancer cells, analyze the corresponding gene sequence, and explore the molecular mechanism of arsenic trioxide on the apoptosis of liver cancer cells.
Methods In this study, arsenic trioxide was used to induce apoptosis of human liver cancer HCC-9204 cells, and the differential expression in apoptotic cells was cloned by using inhibitory subtractive hybridization technology. Comparative analysis of homology characteristics.
Results In this study, 18 cDNA sequences with high homology to known genes were cloned. The types of genome control include anti-oxidative damage, mitochondrial transmembrane transport cytoskeleton and system-related genes.
Conclusion   After arsenic trioxide-induced apoptosis of liver cancer HCC-9204 cells, a large number of gene expressions are up-regulated, and these genes are closely related to the stress response and apoptosis of liver cancer cells after arsenic trioxide treatment. Judging from the results of this study, it is suggested that apoptosis is characterized by the participation of multiple genes, and it is an extremely complex process that is regulated by many gene types.