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Open Access Article

International Journal of Genetics Frontiers. 2020; 1: (1) 5; 24-29 ; DOI: 10.12208/j.ijgf.20200005.

Interleukin-6 (-174 G/C) and Hepcidin HAMP (-582A/G) Gene Polymorphisms among Sudanese Patients with Anemia of Chronic Kidney Disease Treated with Erythropoietin
促红细胞生成素治疗苏丹慢性肾脏病贫血患者的白细胞介素-6(-174G/C)和铁调素HAMP(-582A/G)基因多态性

作者: Khalid Mohamed Khalid Elhussain*, Amged Husssen Abdelrhman, Enaam Abdelrhman Abdelgadir

奥姆杜尔曼伊斯兰大学血液学和免疫血液学系,奥德穆尔曼·阿赫利亚大学医学部,非洲国际大学医学检验科学学院

奥姆杜尔曼伊斯兰大学血液学和免疫血液学系

苏丹铝大学医学院病理学系

*通讯作者: Khalid Mohamed Khalid Elhussain,单位:奥姆杜尔曼伊斯兰大学血液学和免疫血液学系,奥德穆尔曼·阿赫利亚大学医学部,非洲国际大学医学检验科学学院;

发布时间: 2020/6/17 11:29:23 总浏览量: 873

摘要

目的 这是一项病例对照研究,用于通过聚合酶链反应测定苏丹红细胞生成素治疗的慢性肾脏病贫血患者中白细胞介素6(-174 G / C)和铁调素HAMP(-582A / G)基因多态性的频率反应限制片段长度多态性(PCR / RFLP)。结果 RBCS曲线水平显着降低:观察到具有CKD和RBCS曲线的患者的RBC计数,Hb和PCV。结果显示苏丹ACKD患者中IL-6基因和铁调素(HAMP)基因启动子的多态性(SNP)。在Hepcidin HAMP(-582A / G)(SNP)AA基因型70(35%),AG 23(11.5)和GG 7(3.5%)中存在IL-6(-174G / C)(SNP)依赖透析的患者和200名对照组的AA 166(41.5%),AG 34(8.5%)和GG 0(0%),等位基因A在受ACKD影响的患者中更为频繁。

关键词: 贫血;白介素6基因多态性; hepcidin HAMP基因多态性;慢性肾脏病患者

Abstract

Objectives. This is a case controls study used to determine the frequency of Interleukin-6 (-174 G/C) and hepcidin HAMP (-582A/G) gene polymorphisms among Sudanese patients with anemia of chronic kidney disease treated with erythropoietin by using the Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR/RFLP).
Results. Significantly decreased levels in RBCS profile: RBCs count, Hb and PCVin patients with CKD and RBCS profile were observed. The polymorphisms (SNP) of the IL-6 gene and hepcidin (HAMP) gene promoter in Sudanese patients with ACKD were showed. the IL-6 (-174G/C) (SNP) was present in the hepcidin HAMP (-582A/G) (SNP) AA genotype 70 (35%), AG 23 (11.5) and GG 7 (3.5%) in 100 patients dialysis dependent and AA 166 (41.5%), AG 34 (8.5%) and GG 0 (0%) in 200 control subjects, and the allele A are more frequent in patients affected by ACKD.

Key words: Anemia; Interleukin-6 gene polymorphism; hepcidin HAMP gene polymorphism; Chronic kidney disease patients

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引用本文

Khalid Mohamed Khalid Elhussain, Amged Husssen Abdelrhman, Enaam Abdelrhman Abdelgadir, 促红细胞生成素治疗苏丹慢性肾脏病贫血患者的白细胞介素-6(-174G/C)和铁调素HAMP(-582A/G)基因多态性[J]. 国际遗传前沿杂志, 2020; 1: (1) : 24-29.