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Open Access Article

International Journal of Genetics Frontiers. 2020; 1: (1) 2; 5-11 ; DOI: 10.12208/j.ijgf.20200002.

Genetic polymorphism of DNATyper X19 kit in Northern Chinese Han people
DNATyper X19试剂盒在中国北方汉族人的遗传多态性

作者: YU Zhengliang, CAI Huiju, MA Wenhua, MO Xiaoting, YAO Yiren, WANG Le, LIU Chao, ZHAO Xingchun, LI Wanshui*,

公安部法医学研究所

广州法医所

*通讯作者: LI Wanshui,单位:公安部法医学研究所;

发布时间: 2020/6/17 11:26:23 总浏览量: 529

摘要

目的 评估DNATyper X19试剂盒在法医学应用中的价值。方法 在这项研究中,我们对374名中国北方汉族个体的遗传多态性进行了调查。结果 在374名华北汉族人群的18个X-STR基因座中共检测到140个等位基因,从228名男性中检测出228个单倍型,多样性为100%。在这项研究中,应用Bonferroni校正进行多次测试后(p = 0.05 / 18),没有标记脱离Hardy-Weinberg平衡,GATA31E08-DXS6797,DXS8377-DXS10079处于连锁不平衡(LD)(p = 0.05 / 153)。多态信息含量(PIC)为0.434-0.908,判别力(DP)为0.432-0.983。男性的综合分辨力为0.99999993,女性为0.999999992。双重病例(CMECduo)的合并平均排除机会为0.999993,三重病例(CMECtrio)为0.999999994。结论 DNATyper X19试剂盒对复杂的父系案件具有潜在价值。

关键词: 法医遗传学,X染色体,X-STR

Abstract

Objective To evaluate the value of DNATyper X19 kit in the forensic application.
Methods In this study, we carried out a survey of genetic polymorphism from 374 Northern Chinese Han individuals.
Results A total of 140 alleles were detected among the 18 X-STR loci from 374 Northern Chinese Han individuals, 228 haplotypes were detected from the 228 males, the diversity is 100%. No markers departed from Hardy-Weinberg equilibrium after applying Bonferroni’s correction for multiple testing (p=0.05/18), GATA31E08-DXS6797, DXS8377-DXS10079 were in linkage disequilibrium (LD) in this study (p=0.05/153). The polymorphism information content (PIC) was 0.434-0.908 and the discrimination power (DP) was 0.432-0.983. The combined discrimination power was 0.99999993 for male and 0.999999992 for female. The combined mean exclusion chance was 0.999993 in duo cases (CMECduo) and 0.999999994 in trio case (CMECtrio).
Conclusion  s DNATyper X19 kit showed potential value for complicated paternity cases.

Key words: Forensic Genetics, X Chromosome, X-STR

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引用本文

YU Zhengliang, CAI Huiju, MA Wenhua, MO Xiaoting, YAO Yiren, WANG Le, LIU Chao, ZHAO Xingchun, LI Wanshui, DNATyper X19试剂盒在中国北方汉族人的遗传多态性[J]. 国际遗传前沿杂志, 2020; 1: (1) : 5-11.